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DBR1 Polyclonal antibody
DBR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, HepG2 cells, HeLa cells |
Positive IP detected in | mouse brain tissue, HeLa cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16019-1-AP targets DBR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, rat |
Immunogen | DBR1 fusion protein Ag8830 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | debranching enzyme homolog 1 (S. cerevisiae) |
Synonyms | Lariat debranching enzyme, EC:3.1.4.- |
Calculated Molecular Weight | 544 aa, 62 kDa |
Observed Molecular Weight | 70-80 kDa |
GenBank Accession Number | BC009472 |
Gene Symbol | DBR1 |
Gene ID (NCBI) | 51163 |
RRID | AB_2230316 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UK59 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DBR1 antibody 16019-1-AP | Download protocol |
IHC protocol for DBR1 antibody 16019-1-AP | Download protocol |
IF protocol for DBR1 antibody 16019-1-AP | Download protocol |
IP protocol for DBR1 antibody 16019-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Genet Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.
| ||
Mol Cell Proteomics Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition. | ||
PLoS One Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus. | ||
Int J Mol Sci Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein. |