验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HepG2 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue, HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16019-1-AP targets DBR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | DBR1 fusion protein Ag8830 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | debranching enzyme homolog 1 (S. cerevisiae) |
| 别名 | Lariat debranching enzyme, EC:3.1.4.- |
| 计算分子量 | 544 aa, 62 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC009472 |
| 基因名称 | DBR1 |
| Gene ID (NCBI) | 51163 |
| RRID | AB_2230316 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UK59 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DBR1 antibody 16019-1-AP | Download protocol |
| IHC protocol for DBR1 antibody 16019-1-AP | Download protocol |
| IF protocol for DBR1 antibody 16019-1-AP | Download protocol |
| IP protocol for DBR1 antibody 16019-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.
| ||
Mol Cell Proteomics Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition. | ||
PLoS One Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus. | ||
Int J Mol Sci Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein. |
