DCC-Specific Monoclonal antibody
DCC-Specific Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse and More (1)
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2G4G4
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
67203-1-Ig targets DCC-Specific in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | mouse |
| Immunogen | DCC-Specific fusion protein Ag20569 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | deleted in colorectal carcinoma |
| Synonyms | Colorectal cancer suppressor, CRC18, CRCR1, DCC, IGDCC1, Netrin receptor DCC, Tumor suppressor protein DCC |
| Calculated Molecular Weight | 1447 aa, 158 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | NM_005215 |
| Gene Symbol | DCC |
| Gene ID (NCBI) | 1630 |
| RRID | AB_2882496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC plays a key role in the developing nervous system. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCC-Specific antibody 67203-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Novel mechanisms of Anshen Dingzhi prescription against PTSD: Inhibiting DCC to modulate synaptic function and inflammatory responses | ||
Neurobiol Stress DCC, a potential target for controlling fear memory extinction and hippocampal LTP in male mice receiving single prolonged stress |