验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, human lung tissue, human brain tissue, human kidney tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells, BxPC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
11535-1-AP targets DCI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | DCI fusion protein Ag2094 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase) |
别名 | ECI1, EC:5.3.3.8, Dodecenoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase |
计算分子量 | 302 aa, 33 kDa |
观测分子量 | 31 kDa |
GenBank蛋白编号 | BC020228 |
基因名称 | DCI |
Gene ID (NCBI) | 1632 |
RRID | AB_2089906 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P42126 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).
实验方案
Product Specific Protocols | |
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WB protocol for DCI antibody 11535-1-AP | Download protocol |
IHC protocol for DCI antibody 11535-1-AP | Download protocol |
IF protocol for DCI antibody 11535-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |