验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, human lung tissue, human brain tissue, human kidney tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, BxPC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11535-1-AP targets DCI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | DCI fusion protein Ag2094 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase) |
| Synonyms | ECI1, EC:5.3.3.8, Dodecenoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase |
| Calculated Molecular Weight | 302 aa, 33 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC020228 |
| Gene Symbol | DCI |
| Gene ID (NCBI) | 1632 |
| RRID | AB_2089906 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCI antibody 11535-1-AP | Download protocol |
| IHC protocol for DCI antibody 11535-1-AP | Download protocol |
| IF protocol for DCI antibody 11535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
