验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 9 publications below |
产品信息
17758-1-AP targets DCK in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | DCK fusion protein Ag12123 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | deoxycytidine kinase |
别名 | DCK, deoxycytidine kinase |
计算分子量 | 260 aa, 31 kDa |
观测分子量 | 30 kDa |
GenBank蛋白编号 | BC103764 |
基因名称 | DCK |
Gene ID (NCBI) | 1633 |
RRID | AB_2261476 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P27707 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443).
实验方案
Product Specific Protocols | |
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WB protocol for DCK antibody 17758-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncogene KNSTRN promotes tumorigenesis and gemcitabine resistance by activating AKT in bladder cancer. | ||
Int J Cancer Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism. | ||
Front Oncol m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer. | ||
Invest New Drugs Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma. | ||
Sci Rep Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia.
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