- Featured Product
- KD/KO Validated
DCLK1 Polyclonal antibody
DCLK1 Polyclonal Antibody for WB, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF-P, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, human brain tissue, mouse brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21699-1-AP targets DCLK1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF-P, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DCLK1 fusion protein Ag16292 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | doublecortin-like kinase 1 |
| Synonyms | EC:2.7.11.1, Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A |
| Calculated Molecular Weight | 729 aa, 81 kDa |
| Observed Molecular Weight | 48 kDa, 82 kDa |
| GenBank Accession Number | BC152456 |
| Gene Symbol | DCLK1 |
| Gene ID (NCBI) | 9201 |
| RRID | AB_10859251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15075 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCLK1 antibody 21699-1-AP | Download protocol |
| IF protocol for DCLK1 antibody 21699-1-AP | Download protocol |
| IP protocol for DCLK1 antibody 21699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine. | ||
Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response | ||
Clin Transl Med Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.
| ||
J Gastroenterol High-throughput screening identified miR-7-2-3p and miR-29c-3p as metastasis suppressors in gallbladder carcinoma.
| ||
Front Pharmacol XMD-17-51 Inhibits DCLK1 Kinase and Prevents Lung Cancer Progression.
| ||
Cell Physiol Biochem MicroRNA-195 Suppresses the Progression of Pancreatic Cancer by Targeting DCLK1. |
