• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DCLK1 Polyclonal antibody, PBS Only

DCLK1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29800-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    IP experiment of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • WB analysis using 29800-1-AP (same clone as 29800-1-PBS)

    WB analysis using 29800-1-AP (same clone as 29800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

产品信息

29800-1-PBS targets DCLK1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DCLK1 fusion protein Ag32007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublecortin-like kinase 1
Synonyms
Calculated Molecular Weight82KD
Observed Molecular Weight48 kDa, 82 kDa
GenBank Accession NumberNM_004734
Gene Symbol DCLK1
Gene ID (NCBI) 9201
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15075
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.