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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68234-1-Ig (same clone as 68234-1-PBS)

    WB analysis using 68234-1-Ig (same clone as 68234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    IF Staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (68234-1-Ig, Clone: 3C12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • FC experiment of Neuro-2a using 68234-1-Ig (same clone as 68234-1-PBS)

    FC experiment of Neuro-2a using 68234-1-Ig (same clone as 68234-1-PBS)

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human DCLK1 (68234-1-Ig, Clone:3C12C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • WB analysis of fetal human brain using 68234-1-Ig (same clone as 68234-1-PBS)

    WB analysis of fetal human brain using 68234-1-Ig (same clone as 68234-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

DCLK1 Monoclonal antibody, PBS Only

DCLK1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 68234-1-PBS

产品说明书

CloneNo. 3C12C1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit, pig

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A, DCAMKL1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68234-1-PBS targets DCLK1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit, pig
免疫原 DCLK1 fusion protein Ag17110 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 doublecortin-like kinase 1
别名 Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A, DCAMKL1
计算分子量 729 aa, 81 kDa
观测分子量46 kDa, 82 kDa
GenBank蛋白编号BC152456
基因名称 DCLK1
Gene ID (NCBI) 9201
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15075
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.