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DCLK1 Monoclonal antibody, PBS Only

DCLK1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12C1

Cat no : 68234-1-PBS

Print datasheet

Synonyms

Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A, DCAMKL1

验证数据展示

  • WB analysis using 68234-1-Ig (same clone as 68234-1-PBS)

    WB analysis using 68234-1-Ig (same clone as 68234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    IF Staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (68234-1-Ig, Clone: 3C12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • FC experiment of Neuro-2a using 68234-1-Ig (same clone as 68234-1-PBS)

    FC experiment of Neuro-2a using 68234-1-Ig (same clone as 68234-1-PBS)

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human DCLK1 (68234-1-Ig, Clone:3C12C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • WB analysis of fetal human brain using 68234-1-Ig (same clone as 68234-1-PBS)

    WB analysis of fetal human brain using 68234-1-Ig (same clone as 68234-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 68234-1-Ig (DCLK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    IHC staining of mouse brain using 68234-1-Ig (same clone as 68234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68234-1-Ig (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68234-1-PBS in a different storage buffer formulation.

产品信息

68234-1-PBS targets DCLK1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen DCLK1 fusion protein Ag17110 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name doublecortin-like kinase 1
Synonyms Doublecortin-like kinase 1, Doublecortin-like and CAM kinase-like 1, Doublecortin domain-containing protein 3A, DCDC3A, DCAMKL1
Calculated Molecular Weight 729 aa, 81 kDa
Observed Molecular Weight46 kDa, 82 kDa
GenBank Accession NumberBC152456
Gene Symbol DCLK1
Gene ID (NCBI) 9201
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15075
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.