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DCLK2 Polyclonal antibody

DCLK2 Polyclonal Antibody for WB, IP, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 25450-1-AP

Print datasheet

Synonyms

DCLK2, Doublecortin domain-containing protein 3B, Doublecortin-like and CAM kinase-like 2, Doublecortin-like kinase 2, EC:2.7.11.1, Serine/threonine-protein kinase DCLK2, CaMK-like CREB regulatory kinase 2, CL2, CLICK II, CLICK2, CLICK-II, DCAMKL2, DCDC3B, DCK2

验证数据展示

  • WB analysis using 25450-1-AP

    WB analysis using 25450-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25450-1-AP (DCLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 25450-1-AP

    IP experiment of mouse brain using 25450-1-AP

    IP result of anti-DCLK2 (IP:25450-1-AP, 4ug; Detection:25450-1-AP 1:3000) with mouse brain tissue lysate 2100 ug.

  • IF Staining of HepG2 using 25450-1-AP

    IF Staining of HepG2 using 25450-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCLK2 antibody (25450-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25450-1-AP targets DCLK2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DCLK2 fusion protein Ag22142 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublecortin-like kinase 2
Synonyms DCLK2, Doublecortin domain-containing protein 3B, Doublecortin-like and CAM kinase-like 2, Doublecortin-like kinase 2, EC:2.7.11.1, Serine/threonine-protein kinase DCLK2, CaMK-like CREB regulatory kinase 2, CL2, CLICK II, CLICK2, CLICK-II, DCAMKL2, DCDC3B, DCK2
Calculated Molecular Weight 766 aa, 84 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC032726
Gene Symbol DCLK2
Gene ID (NCBI) 166614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N568
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCLK2 belongs to the protein kinase superfamily, contains two N-terminal doublecortin domains, a C-terminal serine/threonine protein kinase domain, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains. DCLK2 is expressed in the brain, heart and eyes (PMID: 18075264).

实验方案

Product Specific Protocols
WB protocol for DCLK2 antibody 25450-1-APDownload protocol
IF protocol for DCLK2 antibody 25450-1-APDownload protocol
IP protocol for DCLK2 antibody 25450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols