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验证数据展示

  • WB analysis using 25450-1-AP

    WB analysis using 25450-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25450-1-AP (DCLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 25450-1-AP

    IP experiment of mouse brain using 25450-1-AP

    IP result of anti-DCLK2 (IP:25450-1-AP, 4ug; Detection:25450-1-AP 1:3000) with mouse brain tissue lysate 2100 ug.

  • IF Staining of HepG2 using 25450-1-AP

    IF Staining of HepG2 using 25450-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCLK2 antibody (25450-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

DCLK2 Polyclonal antibody

DCLK2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 25450-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

DCAMKL2, CLICK-II, CLICK2, CLICK II, CL2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25450-1-AP targets DCLK2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DCLK2 fusion protein Ag22142 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublecortin-like kinase 2
Synonyms DCAMKL2, CLICK-II, CLICK2, CLICK II, CL2
Calculated Molecular Weight 766 aa, 84 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC032726
Gene Symbol DCLK2
Gene ID (NCBI) 166614
RRIDAB_3085796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N568
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCLK2 belongs to the protein kinase superfamily, contains two N-terminal doublecortin domains, a C-terminal serine/threonine protein kinase domain, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains. DCLK2 is expressed in the brain, heart and eyes (PMID: 18075264).

实验方案

Product Specific Protocols
WB protocol for DCLK2 antibody 25450-1-APDownload protocol
IF protocol for DCLK2 antibody 25450-1-APDownload protocol
IP protocol for DCLK2 antibody 25450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols