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验证数据展示

  • WB analysis using 85047-5-RR (same clone as 85047-5-PBS)

    WB analysis using 85047-5-RR (same clone as 85047-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85047-5-RR (DCP1A antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85047-5-RR (same clone as 85047-5-PBS)

    IHC staining of mouse brain using 85047-5-RR (same clone as 85047-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCP1A antibody included
  • IF Staining of HepG2 using 85047-5-RR (same clone as 85047-5-PBS)

    IF Staining of HepG2 using 85047-5-RR (same clone as 85047-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (85047-5-RR, Clone: 242553F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

DCP1A Recombinant monoclonal antibody, PBS Only

DCP1A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85047-5-PBS

产品说明书

CloneNo. 242553F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DCP1A Ready-To-Use IHC Kit. DCP1A primary antibody included.

产品信息

85047-5-PBS targets DCP1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18027

Product name: Recombinant human DCP1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC007439

Sequence: MEALSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDKQSPSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSNLGSTETLEEMPSGSQDKSAPSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAAHHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DCP1 decapping enzyme homolog A (S. cerevisiae)
别名 EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360
计算分子量 582 aa, 63 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC007439
基因名称 DCP1A
Gene ID (NCBI) 55802
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPI6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

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