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DCP1B Polyclonal antibody

DCP1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13863-1-AP

Print datasheet

Synonyms

mRNA-decapping enzyme 1B, hDcp1b, EC:3.6.1.62, DCP1

验证数据展示

  • WB analysis using 13863-1-AP

    WB analysis using 13863-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13863-1-AP (DCP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13863-1-AP

    IHC staining of human liver cancer using 13863-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 13863-1-AP

    IHC staining of human liver cancer using 13863-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13863-1-AP (DCP1B antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HeLa using 13863-1-AP

    IP experiment of HeLa using 13863-1-AP

    IP result of anti-DCP1B (IP:13863-1-AP, 4ug; Detection:13863-1-AP 1:500) with HeLa cells lysate 1520ug.

  • IF Staining of HeLa using 13863-1-AP

    IF Staining of HeLa using 13863-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCP1B antibody (13863-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells, human liver tissue, Jurkat cells, rat liver tissue, rat spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13863-1-AP targets DCP1B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Immunogen DCP1B fusion protein Ag4845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DCP1 decapping enzyme homolog B (S. cerevisiae)
Synonyms mRNA-decapping enzyme 1B, hDcp1b, EC:3.6.1.62, DCP1
Calculated Molecular Weight 618 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC043437
Gene Symbol DCP1B
Gene ID (NCBI) 196513
RRIDAB_2090411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZD4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCP1B belongs to DCP1 family. DCP1B may play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. It may remove the 7-methyl guanine cap structure mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP.  DCP1B has 2 isoforms with the molecular mass of 56 and 68 kDa. DCP1B is associated with DM-related atherogenesis (PMID:33195466).

实验方案

Product Specific Protocols
WB protocol for DCP1B antibody 13863-1-APDownload protocol
IHC protocol for DCP1B antibody 13863-1-APDownload protocol
IF protocol for DCP1B antibody 13863-1-APDownload protocol
IP protocol for DCP1B antibody 13863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Int J Mol Med

LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication.

Authors - Yangchao Dong
View Article