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验证数据展示

  • WB analysis using 17744-1-AP

    WB analysis using 17744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17744-1-AP (DCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 17744-1-AP

    IHC staining of human ovary cancer using 17744-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17744-1-AP (DCP2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DCP2 Polyclonal antibody

DCP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17744-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Nudix motif 20, Nucleoside diphosphate-linked moiety X motif 20, mRNA-decapping enzyme 2, m7GpppN-mRNA hydrolase, hDpc

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Caco-2 cells, T-47D cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17744-1-AP targets DCP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 DCP2 fusion protein Ag12001 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DCP2 decapping enzyme homolog (S. cerevisiae)
别名 Nudix motif 20, Nucleoside diphosphate-linked moiety X motif 20, mRNA-decapping enzyme 2, m7GpppN-mRNA hydrolase, hDpc
计算分子量385aa,44 kDa; 420aa,48 kDa
观测分子量48 kDa
GenBank蛋白编号BC064593
基因名称 DCP2
Gene ID (NCBI) 167227
RRIDAB_3085538
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IU60
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DCP2 antibody 17744-1-APDownload protocol
IHC protocol for DCP2 antibody 17744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols