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  • Featured Product
  • KD/KO Validated

DCTN4 Polyclonal antibody

DCTN4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17098-1-AP

Print datasheet

Synonyms

Dynactin subunit p62, Dynactin subunit 4, Dyn4

验证数据展示

  • WB analysis of human brain using 17098-1-AP

    WB analysis of human brain using 17098-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 17098-1-AP

    IP experiment of Jurkat using 17098-1-AP

    IP result of anti-DCTN4 (IP:17098-1-AP, 4ug; Detection:17098-1-AP 1:700) with Jurkat cells lysate 2800ug.

  • IHC staining of human stomach using 17098-1-AP

    IHC staining of human stomach using 17098-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17098-1-AP (DCTN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17098-1-AP targets DCTN4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, monkey
Immunogen DCTN4 fusion protein Ag10579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynactin 4 (p62)
Synonyms Dynactin subunit p62, Dynactin subunit 4, Dyn4
Calculated Molecular Weight 460 aa, 52 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC026323
Gene Symbol DCTN4
Gene ID (NCBI) 51164
RRIDAB_2276944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DCTN4 antibody 17098-1-APDownload protocol
IHC protocol for DCTN4 antibody 17098-1-APDownload protocol
IP protocol for DCTN4 antibody 17098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
MonkeyWB

Mol Biol Cell

Dynactin's pointed-end complex is a cargo-targeting module.

Authors - Ting-Yu Yeh
  • KD Validated
View Article
humanWB

Onco Targets Ther

Linc01094 Accelerates the Growth and Metastatic-Related Traits of Glioblastoma by Sponging miR-126-5p.

Authors - Xin Xing Li
View Article