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DCTPP1 Polyclonal antibody

DCTPP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16684-1-AP

Print datasheet

Synonyms

CDA03, dCTP pyrophosphatase 1, dCTPase 1, DCTPP1, Deoxycytidine triphosphatase 1, RS21C6, XTP3TPA

验证数据展示

  • WB analysis of HeLa using 16684-1-AP

    WB analysis of HeLa using 16684-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 16684-1-AP

    IHC staining of human lung cancer using 16684-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCTPP1 antibody included
  • IF Staining of HeLa using 16684-1-AP

    IF Staining of HeLa using 16684-1-AP

    Immunofluorescent analysis of HeLa cells using 16684-1-AP (DCTPP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung cancer using 16684-1-AP

    IHC staining of human lung cancer using 16684-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCTPP1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, Transfected HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DCTPP1 Ready-To-Use IHC Kit. DCTPP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16684-1-AP targets DCTPP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DCTPP1 fusion protein Ag10015 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dCTP pyrophosphatase 1
Synonyms CDA03, dCTP pyrophosphatase 1, dCTPase 1, DCTPP1, Deoxycytidine triphosphatase 1, RS21C6, XTP3TPA
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa, 40 kDa, 80 kDa
GenBank Accession NumberBC001344
Gene Symbol DCTPP1
Gene ID (NCBI) 79077
RRIDAB_2878299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H773
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCTPP1, also named as XTP3TPA, CDAO3 and RS21C6, is a hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. It has a central role in the balance of dCTP and the metabolism of deoxycytidine analogues, thus contributing to the preservation of genome integrity. DCTPP1 has some forms such as monomer (~20 kDa), Dimer (~40 kDa) and Homotetramer (~80 kDa).

实验方案

Product Specific Protocols
WB protocol for DCTPP1 antibody 16684-1-APDownload protocol
IHC protocol for DCTPP1 antibody 16684-1-APDownload protocol
IF protocol for DCTPP1 antibody 16684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Cell Mol Med

Expression of human dCTP pyrophosphatase 1 (DCTPP1) and its association with cisplatin resistance characteristics in ovarian cancer

Authors - Yu Wang
View Article