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验证数据展示

  • WB analysis of MCF-7 using 26356-1-AP

    WB analysis of MCF-7 using 26356-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26356-1-AP (DCUN1D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DCUN1D3 Polyclonal antibody

DCUN1D3 Polyclonal Antibody for WB, ELISA
Cat No. 26356-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

44M2.4, DCN1 like protein 3, DCUN1D3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy DCUN1D3 Ready-To-Use IHC Kit. DCUN1D3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26356-1-AP targets DCUN1D3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 DCUN1D3 fusion protein Ag23730 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae)
别名 44M2.4, DCN1 like protein 3, DCUN1D3
观测分子量 34 kDa
GenBank蛋白编号BC040442
基因名称 DCUN1D3
Gene ID (NCBI) 123879
RRIDAB_2880489
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWE4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCUN1D3, also named as SCCRO3, is proposed to function as a tumor suppressor. DCUN1D3 antagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane (PubMed:25349211). When overexpressed in transformed cells, DCUN1D3 may promote mesenchymal to epithelial-like changes and inhibit colony formation in soft agar (PubMed:25349211).

实验方案

Product Specific Protocols
WB protocol for DCUN1D3 antibody 26356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols