验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse liver tissue, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DDA1 Ready-To-Use IHC Kit. DDA1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14995-1-AP targets DDA1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | DDA1 fusion protein Ag6981 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DET1 and DDB1 associated 1 |
| 别名 | Placenta cross-immune reaction antigen 1, PCIA-1, PCIA1, DET1- and DDB1-associated protein 1, C19orf58 |
| 计算分子量 | 12 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC000615 |
| 基因名称 | DDA1 |
| Gene ID (NCBI) | 79016 |
| RRID | AB_10859095 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW61 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDA1, also known as C19orf58 and PCIA1, belongs to the DDA1 family. DDA1 is an evolutionarily conserved gene located at 19p13.11 that may be involved in the activation of nuclear factor kappaB (NFκB). DDA1 encodes an 12 kDa protein with 102 AA residues whose orthologs share 82-92% identity with Arabidopsis, invertebrates, and vertebrates. Additionally, DDA1 might be involved in tumour formation and progression (PMID: 26942699, 28211159).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDA1 antibody 14995-1-AP | Download protocol |
| IHC protocol for DDA1 antibody 14995-1-AP | Download protocol |
| IF protocol for DDA1 antibody 14995-1-AP | Download protocol |
| IP protocol for DDA1 antibody 14995-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. | ||
Nat Chem Biol Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.
| ||
Curr Biol Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism. | ||
EMBO Mol Med A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. | ||
