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DDA1 Polyclonal antibody

DDA1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14995-1-AP

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Synonyms

Placenta cross-immune reaction antigen 1, PCIA-1, PCIA1, DET1- and DDB1-associated protein 1, C19orf58



经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDA1 Ready-To-Use IHC Kit. DDA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14995-1-AP targets DDA1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen DDA1 fusion protein Ag6981 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DET1 and DDB1 associated 1
Synonyms Placenta cross-immune reaction antigen 1, PCIA-1, PCIA1, DET1- and DDB1-associated protein 1, C19orf58
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC000615
Gene Symbol DDA1
Gene ID (NCBI) 79016
RRIDAB_10859095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW61
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDA1, also known as C19orf58 and PCIA1, belongs to the DDA1 family. DDA1 is an evolutionarily conserved gene located at 19p13.11 that may be involved in the activation of nuclear factor kappaB (NFκB). DDA1 encodes an 12 kDa protein with 102 AA residues whose orthologs share 82-92% identity with Arabidopsis, invertebrates, and vertebrates. Additionally, DDA1 might be involved in tumour formation and progression (PMID: 26942699, 28211159).

实验方案

Product Specific Protocols
WB protocol for DDA1 antibody 14995-1-APDownload protocol
IHC protocol for DDA1 antibody 14995-1-APDownload protocol
IF protocol for DDA1 antibody 14995-1-APDownload protocol
IP protocol for DDA1 antibody 14995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

CRL4AMBRA1 is a master regulator of D-type cyclins.

Authors - Daniele Simoneschi
humanWB

Science

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Authors - Ting Han
humanWB

Nat Chem Biol

Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.

Authors - Dirksen E Bussiere
  • KD Validated
mouseIP, WB

Curr Biol

Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.

Authors - Luca Rizzini
humanIHC,IF

EMBO Mol Med

A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.

Authors - Lucía Zhu
humanWB

Cell Rep

Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.

Authors - Tabitha C Ting