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DDAH1 Polyclonal antibody

DDAH1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30108-1-AP

Print datasheet

Synonyms

Dimethylargininase 1, DDAHI, DDAH 1, DDAH

验证数据展示

  • WB analysis using 30108-1-AP

    WB analysis using 30108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30108-1-AP (DDAH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30108-1-AP

    IHC staining of mouse brain using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 30108-1-AP

    IF Staining of mouse eye using 30108-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using DDAH1 antibody (30108-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 30108-1-AP

    IHC staining of human stomach cancer using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30108-1-AP

    IHC staining of mouse brain using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30108-1-AP

    IHC staining of rat brain using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCaco-2 cells, SH-SY5Y cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human stomach cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30108-1-AP targets DDAH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DDAH1 fusion protein Ag32516 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dimethylarginine dimethylaminohydrolase 1
Synonyms Dimethylargininase 1, DDAHI, DDAH 1, DDAH
Calculated Molecular Weight31kd
Observed Molecular Weight35 kDa
GenBank Accession NumberNM_012137
Gene Symbol DDAH1
Gene ID (NCBI) 23576
RRIDAB_2935515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is an enzyme that can degrade asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS)inhibitor (PMID:26996393). About 80% of endogenous ADMA is metabolized by DDAH, principally the DDAH1 isoform (PMID:22460174). It was reported that DDAH1 is highly expressed in vascular endothelial cells in hearts (PMID:19917889). The observed molecular weight of DDAH1 is 35-40 kDa in the literature.

实验方案

Product Specific Protocols
WB protocol for DDAH1 antibody 30108-1-APDownload protocol
IHC protocol for DDAH1 antibody 30108-1-APDownload protocol
IF protocol for DDAH1 antibody 30108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols