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验证数据展示

  • WB analysis using 30108-1-AP

    WB analysis using 30108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30108-1-AP (DDAH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30108-1-AP

    IHC staining of mouse brain using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 30108-1-AP

    IF Staining of mouse eye using 30108-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using DDAH1 antibody (30108-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 30108-1-AP

    IHC staining of human stomach cancer using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30108-1-AP

    IHC staining of mouse brain using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30108-1-AP

    IHC staining of rat brain using 30108-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30108-1-AP (DDAH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DDAH1 Polyclonal antibody

DDAH1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 30108-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Dimethylargininase 1, DDAHI, DDAH 1, DDAH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, SH-SY5Y cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human stomach cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30108-1-AP targets DDAH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDAH1 fusion protein Ag32516 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dimethylarginine dimethylaminohydrolase 1
别名 Dimethylargininase 1, DDAHI, DDAH 1, DDAH
计算分子量31kd
观测分子量35 kDa
GenBank蛋白编号NM_012137
基因名称 DDAH1
Gene ID (NCBI) 23576
RRIDAB_2935515
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94760
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is an enzyme that can degrade asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS)inhibitor (PMID:26996393). About 80% of endogenous ADMA is metabolized by DDAH, principally the DDAH1 isoform (PMID:22460174). It was reported that DDAH1 is highly expressed in vascular endothelial cells in hearts (PMID:19917889). The observed molecular weight of DDAH1 is 35-40 kDa in the literature.

实验方案

Product Specific Protocols
WB protocol for DDAH1 antibody 30108-1-APDownload protocol
IHC protocol for DDAH1 antibody 30108-1-APDownload protocol
IF protocol for DDAH1 antibody 30108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols