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  • Featured Product
  • KD/KO Validated

DDB1 Polyclonal antibody, PBS Only

DDB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11380-1-PBS

Print datasheet

Synonyms

UV DDB1, HBV X-associated protein 1, DNA damage-binding protein a, DNA damage-binding protein 1, DDBA

验证数据展示

  • WB analysis using 11380-1-AP (same clone as 11380-1-PBS)

    WB analysis using 11380-1-AP (same clone as 11380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDB1 antibody included
  • IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDB1 antibody included
  • IP experiment of Jurkat using 11380-1-AP (same clone as 11380-1-PBS)

    IP experiment of Jurkat using 11380-1-AP (same clone as 11380-1-PBS)

    IP result of anti-DDB1 (IP:11380-1-AP, 5ug; Detection:11380-1-AP 1:500) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    IHC staining of human colon cancer using 11380-1-AP (same clone as 11380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11380-1-AP (DDB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDB1 antibody included
Planning an IHC experiment? We recommend our IHCeasy DDB1 Ready-To-Use IHC Kit. DDB1 primary antibody included.

产品信息

11380-1-PBS targets DDB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DDB1 fusion protein Ag1901 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name damage-specific DNA binding protein 1, 127kDa
Synonyms UV DDB1, HBV X-associated protein 1, DNA damage-binding protein a, DNA damage-binding protein 1, DDBA
Calculated Molecular Weight 1140 aa, 127 kDa
Observed Molecular Weight 127 kDa
GenBank Accession NumberBC011686
Gene Symbol DDB1
Gene ID (NCBI) 1642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16531
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.