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DDHD1 Polyclonal antibody

DDHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26297-1-AP

Print datasheet

Synonyms

Phosphatidic acid-preferring phospholipase A1 homolog, PA-PLA1, KIAA1705, EC:3.1.1.118, EC:3.1.1.111

验证数据展示

  • WB analysis using 26297-1-AP

    WB analysis using 26297-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26297-1-AP (DDHD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 26297-1-AP

    IP experiment of mouse brain using 26297-1-AP

    IP result of anti-DDHD1 (IP:26297-1-AP, 4ug; Detection:26297-1-AP 1:800) with mouse brain tissue lysate 1680 ug.

  • IF Staining of HEK-293 using 26297-1-AP

    IF Staining of HEK-293 using 26297-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DDHD1 antibody (26297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse brain using 26297-1-AP

    IHC staining of mouse brain using 26297-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26297-1-AP (DDHD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26297-1-AP targets DDHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DDHD1 fusion protein Ag23620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DDHD domain containing 1
Synonyms Phosphatidic acid-preferring phospholipase A1 homolog, PA-PLA1, KIAA1705, EC:3.1.1.118, EC:3.1.1.111
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC030703
Gene Symbol DDHD1
Gene ID (NCBI) 80821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEL9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDHD1, also known as KIAA1705, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD1 is highly expressed in the brain and testes. DDHD1 negatively controls the formation of a local phosphatidic acid-rich domain in recycling endosomes that serves as a membrane source for neurite outgrowth (PMID: 32850804).

实验方案

Product Specific Protocols
WB protocol for DDHD1 antibody 26297-1-APDownload protocol
IHC protocol for DDHD1 antibody 26297-1-APDownload protocol
IF protocol for DDHD1 antibody 26297-1-APDownload protocol
IP protocol for DDHD1 antibody 26297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols