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验证数据展示

  • WB analysis of mouse ovary using 12094-1-AP

    WB analysis of mouse ovary using 12094-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12094-1-AP (DDIT4L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DDIT4L Polyclonal antibody

DDIT4L Polyclonal Antibody for WB, ELISA
Cat No. 12094-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, ELISA and More (1)

别名

DNA damage-inducible transcript 4-like protein, HIF-1 responsive protein RTP801-like, REDD2, REDD-2, RTP801L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse ovary tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12094-1-AP targets DDIT4L in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 mouse
文献引用反应性mouse
免疫原 DDIT4L fusion protein Ag2735 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA-damage-inducible transcript 4-like
别名 DNA damage-inducible transcript 4-like protein, HIF-1 responsive protein RTP801-like, REDD2, REDD-2, RTP801L
计算分子量 193 aa, 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC013592
基因名称 DDIT4L
Gene ID (NCBI) 115265
RRIDAB_2918027
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96D03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-damage-inducible transcript 4-like protein(DDIT4L), encoded by the stress responsive gene REDD2, is a negative regulator of mTOR signaling, and expressed predominantly in skeletal muscle. It regulates the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Also, DDIT4L involves in oxidized low-density lipoprotein-induced macrophage death sensitivity.

实验方案

Product Specific Protocols
WB protocol for DDIT4L antibody 12094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

J Cell Sci

Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus-oocyte complexes.

Authors - Jing Guo
View Article
mouseWB

Am J Physiol Endocrinol Metab

Disruption of REDD1 gene ameliorates sepsis-induced decrease in mTORC1 signaling but has divergent effects on proteolytic signaling in skeletal muscle.

Authors - Jennifer L Steiner
View Article