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DDIT4L Polyclonal antibody

DDIT4L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28490-1-AP

Print datasheet

Synonyms

RTP801L, REDD-2, REDD2, HIF-1 responsive protein RTP801-like, DNA damage-inducible transcript 4-like protein

验证数据展示

  • WB analysis using 28490-1-AP

    WB analysis using 28490-1-AP

    Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 28490-1-AP (DDIT4L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse ovary tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28490-1-AP targets DDIT4L in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DDIT4L fusion protein Ag29616 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA-damage-inducible transcript 4-like
Synonyms RTP801L, REDD-2, REDD2, HIF-1 responsive protein RTP801-like, DNA damage-inducible transcript 4-like protein
Calculated Molecular Weight 193 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC013592
Gene Symbol DDIT4L
Gene ID (NCBI) 115265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96D03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-damage-inducible transcript 4-like protein(DDIT4L), encoded by the stress responsive gene REDD2, is a negative regulator of mTOR signaling, and expressed predominantly in skeletal muscle. It regulates the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Also, DDIT4L involves in oxidized low-density lipoprotein-induced macrophage death sensitivity.

实验方案

Product Specific Protocols
WB protocol for DDIT4L antibody 28490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols