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验证数据展示

  • WB analysis using 84438-6-RR

    WB analysis using 84438-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84438-6-RR (DDR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84438-6-RR

    IHC staining of mouse brain using 84438-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84438-6-RR (DDR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDR1 antibody included

DDR1 Recombinant antibody

DDR1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84438-6-RR

Print datasheet

CloneNo. 241796D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Cell adhesion kinase, CD167a, CD167 antigen-like family member A, CD167, CAK

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DDR1 Ready-To-Use IHC Kit. DDR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84438-6-RR targets DDR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name discoidin domain receptor tyrosine kinase 1
Synonyms Cell adhesion kinase, CD167a, CD167 antigen-like family member A, CD167, CAK
Calculated Molecular Weight101kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberNM_001297654.2
Gene Symbol DDR1
Gene ID (NCBI) 780
RRIDAB_3671964
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ08345-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDR1, or Discoidin Domain Receptor 1, is a member of the receptor tyrosine kinase (RTK) family, which plays a significant role in various cellular processes including cell proliferation, adhesion, migration, and extracellular matrix (ECM) remodeling. DDR1 is known for its unique discoidin domain that allows it to bind specifically to certain collagens, initiating downstream signaling pathways that can lead to cell transformation and tumor progression. Abnormal activation of DDR1 is closely associated with the development of various solid tumors, and it has been shown that DDR1 can prevent immune cells from infiltrating triple-negative breast cancer (TNBC) and eliminate tumor cells.

实验方案

Product Specific Protocols
WB protocol for DDR1 antibody 84438-6-RRDownload protocol
IHC protocol for DDR1 antibody 84438-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols