验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293T cells, HEK-293 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19910-1-AP targets DDX17 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, PLA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DDX17 fusion protein Ag13723 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 |
| 别名 | Probable ATP-dependent RNA helicase DDX17, EC:3.6.4.13, DEAD box protein p82, DEAD box protein p72, DEAD box protein 17 |
| 计算分子量 | 729 aa, 80 kDa |
| 观测分子量 | 72-80 kDa |
| GenBank蛋白编号 | BC000595 |
| 基因名称 | DDX17 |
| Gene ID (NCBI) | 10521 |
| RRID | AB_10667004 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92841 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX17, also named as DEAD box protein p72 and DEAD box protein p82, is a 729 amino acid protein, which belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. DDX17 is widely expressed. Levels tend to increase during colon cancer progression, from very low in benign hyperplastic polyps to very high in tubular and villous adenomas. DDX17 as an RNA helicase unwinds RNA and alters RNA structures through ATP binding and hydrolysis. DDX17 is involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. It Regulates the alternative splicing of exons exhibiting specific features. identification and characterisation of p72, a novel human nuclear DEAD box protein, which shows a striking homology to p68. a predicted molecular mass of DDX17 is 72 kDa, but endogenous DDX17 migrates aberrantly at 79 kDa on SDS-PAGE (PMID: 8871553).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX17 antibody 19910-1-AP | Download protocol |
| IHC protocol for DDX17 antibody 19910-1-AP | Download protocol |
| IF protocol for DDX17 antibody 19910-1-AP | Download protocol |
| IP protocol for DDX17 antibody 19910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1. | ||
Nucleic Acids Res The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. | ||
Nucleic Acids Res RNA helicase-dependent gene looping impacts messenger RNA processing
| ||
Clin Transl Med DEAD-Box Helicase 17 exacerbates non-alcoholic steatohepatitis via transcriptional repression of cyp2c29, inducing hepatic lipid metabolism disorder and eliciting the activation of M1 macrophages | ||
PLoS Pathog Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production. | ||
J Biol Chem RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression. |
