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DDX18 Polyclonal antibody

DDX18 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28502-1-AP

Print datasheet

Synonyms

DDX18, DEAD box protein 18, FLJ33908, MrDb, Myc regulated DEAD box protein

验证数据展示

  • WB analysis using 28502-1-AP

    WB analysis using 28502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28502-1-AP (DDX18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 28502-1-AP

    IP experiment of HEK-293T using 28502-1-AP

    IP result of anti-DDX18 (IP:28502-1-AP, 4ug; Detection:28502-1-AP 1:1500) with HEK-293T cells lysate 1560 ug.

  • IF Staining of HEK-293 using 28502-1-AP

    IF Staining of HEK-293 using 28502-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX18 antibody (28502-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293T cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28502-1-AP targets DDX18 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen DDX18 fusion protein Ag27457 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Synonyms DDX18, DEAD box protein 18, FLJ33908, MrDb, Myc regulated DEAD box protein
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC001238
Gene Symbol DDX18
Gene ID (NCBI) 8886
RRIDAB_2881158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX18 antibody 28502-1-APDownload protocol
IF protocol for DDX18 antibody 28502-1-APDownload protocol
IP protocol for DDX18 antibody 28502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols