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验证数据展示

  • WB analysis using 28502-1-AP

    WB analysis using 28502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28502-1-AP (DDX18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 28502-1-AP

    IP experiment of HEK-293T using 28502-1-AP

    IP result of anti-DDX18 (IP:28502-1-AP, 4ug; Detection:28502-1-AP 1:1500) with HEK-293T cells lysate 1560 ug.

  • IF Staining of HEK-293 using 28502-1-AP

    IF Staining of HEK-293 using 28502-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX18 antibody (28502-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

DDX18 Polyclonal antibody

DDX18 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 28502-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

DDX18, DEAD box protein 18, FLJ33908, MrDb, Myc regulated DEAD box protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293T cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28502-1-AP targets DDX18 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse
免疫原 DDX18 fusion protein Ag27457 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
别名 DDX18, DEAD box protein 18, FLJ33908, MrDb, Myc regulated DEAD box protein
计算分子量 75 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC001238
基因名称 DDX18
Gene ID (NCBI) 8886
RRIDAB_2881158
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVP1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX18 antibody 28502-1-APDownload protocol
IF protocol for DDX18 antibody 28502-1-APDownload protocol
IP protocol for DDX18 antibody 28502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols