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DDX19A Polyclonal antibody

DDX19A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15209-1-AP

Print datasheet

Synonyms

DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A

验证数据展示

  • WB analysis using 15209-1-AP

    WB analysis using 15209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15209-1-AP (DDX19A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15209-1-AP

    IHC staining of human ovary tumor using 15209-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15209-1-AP (DDX19A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 15209-1-AP

    IHC staining of human ovary tumor using 15209-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15209-1-AP (DDX19A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15209-1-AP targets DDX19A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen DDX19A fusion protein Ag7362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19A
Synonyms DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A
Calculated Molecular Weight 54 kDa
Observed Molecular Weight44 kDa and 54 kDa
GenBank Accession NumberBC005162
Gene Symbol DDX19A
Gene ID (NCBI) 55308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). DDX19A has 2 isoforms with the molecular mass of 44 and 54 kDa. DDX19A may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

实验方案

Product Specific Protocols
WB protocol for DDX19A antibody 15209-1-APDownload protocol
IHC protocol for DDX19A antibody 15209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

DDX19A Senses Viral RNA and Mediates NLRP3-Dependent Inflammasome Activation.

Authors - Jiangnan Li
View Article