验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
18285-1-AP targets DDX19B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | DDX19B fusion protein Ag13165 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-As) box polypeptide 19B |
| 别名 | DBP5, DDX19, DDX19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, RNAh |
| 计算分子量 | 479aa,54 kDa; 370aa,42 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC010008 |
| 基因名称 | DDX19B |
| Gene ID (NCBI) | 11269 |
| RRID | AB_10597088 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UMR2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX19B antibody 18285-1-AP | Download protocol |
| IP protocol for DDX19B antibody 18285-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
