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DDX20 Polyclonal antibody
DDX20 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, Jurkat cells, mouse testis tissue, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, Hela cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11324-1-AP targets DDX20 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | DDX20 fusion protein Ag1863 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 |
Synonyms | Gemin 3, EC:3.6.1.15, DP103, DEAD box protein DP 103, DEAD box protein 20 |
Calculated Molecular Weight | 824 aa, 92 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC011556 |
Gene Symbol | DDX20 |
Gene ID (NCBI) | 11218 |
RRID | AB_2092401 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UHI6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DDX20 antibody 11324-1-AP | Download protocol |
IHC protocol for DDX20 antibody 11324-1-AP | Download protocol |
IF protocol for DDX20 antibody 11324-1-AP | Download protocol |
IP protocol for DDX20 antibody 11324-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Clin Invest DEAD-box helicase DP103 defines metastatic potential of human breast cancers.
| ||
Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice | ||
Aging (Albany NY) An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes | ||
Comput Struct Biotechnol J Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer. | ||
Arthritis Res Ther Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies. | ||
Biosci Rep FOXL2 Down-regulates Vitellogenin Expression at Mature Stage in Eriocheir sinensis. |