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DDX21 Polyclonal antibody

DDX21 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (4)

Conjugate

Unconjugated

Publications

Cat no : 10528-1-AP

Print datasheet

Synonyms

Gu-alpha, GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21

验证数据展示

WB analysis using 10528-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 10528-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10528-1-AP (DDX21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis using 10528-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10528-1-AP
WB result of DDX21 antibody (10528-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells.
WB analysis using 10528-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 10528-1-AP
IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:1000) with COLO 320 cells lysate 2800ug.
IP experiment of COLO 320 using 10528-1-AP
IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:15000) with COLO 320 cells lysate 2400 ug.
IF Staining of HeLa using 10528-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX21 antibody (10528-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 10528-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DDX21 (10528-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 10528-1-AP

Size

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经过测试的应用

Positive WB detected in	COLO 320 cells, HeLa cells, Jurkat cells, PC-3 cells, HepG2 cells
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 7 publications below
WB	See 26 publications below
IHC	See 5 publications below
IF	See 10 publications below
IP	See 2 publications below
CoIP	See 3 publications below
ChIP	See 1 publications below
RIP	See 1 publications below

产品信息

10528-1-AP targets DDX21 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, chIP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	DDX21 fusion protein Ag0804 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Synonyms	Gu-alpha, GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21
Calculated Molecular Weight	87 kDa
Observed Molecular Weight	87 kDa
GenBank Accession Number	BC008071
Gene Symbol	DDX21
Gene ID (NCBI)	9188
RRID	AB_2092705
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NR30
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

DX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. Catalog# 10528-1-AP is a rabbit polyclonal antibody raised against N-terminal of human DDX21.

实验方案

Product Specific Protocols
WB protocol for DDX21 antibody 10528-1-AP	Download protocol
IHC protocol for DDX21 antibody 10528-1-AP	Download protocol
IF protocol for DDX21 antibody 10528-1-AP	Download protocol
IP protocol for DDX21 antibody 10528-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	CoIP	Science lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription. Authors - Man Wu View Article
human	WB,IF,chIP	Cell SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Authors - Yu-Hang Xing KO Validated View Article
human	WB,IHC,IF	Mol Cell Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21. Authors - Dae-Seok Kim View Article
mouse	WB	Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice Authors - Dingfeng Zou View Article
human	IP	Dev Cell PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation. Authors - Jeanette A Johansson View Article
human	WB,IF	Cancer Res The roles of RNA helicases in DNA damage repair and tumorigenesis reveal precision therapeutic strategies. Authors - Jinru Xie KD Validated View Article

DDX21 Polyclonal antibody

DDX21 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 10528-1-AP

IHC staining of human colon cancer using 10528-1-AP

WB analysis using 10528-1-AP

WB analysis of HeLa using 10528-1-AP

WB analysis using 10528-1-AP

IP experiment of COLO 320 using 10528-1-AP

IP experiment of COLO 320 using 10528-1-AP

IF Staining of HeLa using 10528-1-AP

FC experiment of HepG2 using 10528-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.

The roles of RNA helicases in DNA damage repair and tumorigenesis reveal precision therapeutic strategies.

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