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DDX21 Recombinant antibody, PBS Only

DDX21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E7

Cat no : 80932-1-PBS

Print datasheet

Synonyms

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

验证数据展示

  • WB analysis using 80932-1-RR (same clone as 80932-1-PBS)

    WB analysis using 80932-1-RR (same clone as 80932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80932-1-RR (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80932-1-RR

    Affinity and Kinetic Characterization of 80932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80932-1-RR against Human DDX21 were performed. The affinity constant is 77.7 nM.

产品信息

80932-1-PBS targets DDX21 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen DDX21 fusion protein Ag0804 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Synonyms GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC008071
Gene Symbol DDX21
Gene ID (NCBI) 9188
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NR30
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa.