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验证数据展示

  • FC experiment of U-2 OS using 84103-6-RR

    FC experiment of U-2 OS using 84103-6-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug DDX24 Recombinant antibody (84103-6-RR, Clone:241145G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • WB analysis using 84103-6-RR

    WB analysis using 84103-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84103-6-RR (DDX24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84103-6-RR

    IF Staining of HeLa using 84103-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (84103-6-RR, Clone: 241145G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84103-6-RR

    Affinity and Kinetic Characterization of 84103-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84103-6-RR against Human DDX24 were performed. The affinity constant is below 1 pM.

DDX24 Recombinant antibody

DDX24 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84103-6-RR

产品说明书

CloneNo. 241145G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, HeLa cells, COLO 320 cells, A549 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-2 OS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84103-6-RR targets DDX24 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 DDX24 fusion protein Ag8407 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
别名 EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10
计算分子量 96 kDa
观测分子量 120 kDa
GenBank蛋白编号BC008847
基因名称 DDX24
Gene ID (NCBI) 57062
RRIDAB_3671669
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9GZR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.

实验方案

Product Specific Protocols
WB protocol for DDX24 antibody 84103-6-RRDownload protocol
IF protocol for DDX24 antibody 84103-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols