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DDX27 Polyclonal antibody

DDX27 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17087-1-AP

Print datasheet

Synonyms

Probable ATP-dependent RNA helicase DDX27, PP3241, HSPC259, EC:3.6.4.13, DEAD box protein 27

验证数据展示

  • WB analysis of HeLa using 17087-1-AP

    WB analysis of HeLa using 17087-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17087-1-AP

    IF Staining of HeLa using 17087-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17087-1-AP (DDX27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HEK-293 using 17087-1-AP

    IF Staining of HEK-293 using 17087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX27 antibody (17087-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, NIH/3T3 cells
Positive IF/ICC detected inHEK-293 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17087-1-AP targets DDX27 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DDX27 fusion protein Ag9426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
Synonyms Probable ATP-dependent RNA helicase DDX27, PP3241, HSPC259, EC:3.6.4.13, DEAD box protein 27
Calculated Molecular Weight 796 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC016060
Gene Symbol DDX27
Gene ID (NCBI) 55661
RRIDAB_2092569
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX27 is one of the DEAD box proteins, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DEAD box proteins are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division, and DDX27 is involved in the processing of 5.8S and 28S ribosomal RNAs.

实验方案

Product Specific Protocols
WB protocol for DDX27 antibody 17087-1-APDownload protocol
IF protocol for DDX27 antibody 17087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.

Authors - Francesca Rossi
View Article
humanWB

Onco Targets Ther

DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells.

Authors - Chunxing Yang
View Article