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DDX28 Polyclonal antibody

DDX28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11617-1-AP

Print datasheet

Synonyms

DDX28, MDDX28

验证数据展示

  • WB analysis using 11617-1-AP

    WB analysis using 11617-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11617-1-AP (DDX28 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11617-1-AP

    IHC staining of human prostate cancer using 11617-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11617-1-AP (DDX28 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHEK-293T cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11617-1-AP targets DDX28 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen DDX28 fusion protein Ag2194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
Synonyms DDX28, MDDX28
Calculated Molecular Weight 540 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC024273
Gene Symbol DDX28
Gene ID (NCBI) 55794
RRIDAB_2092574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX28 antibody 11617-1-APDownload protocol
IHC protocol for DDX28 antibody 11617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.

Authors - Christopher Lowden
View Article