DDX4,VASA Polyclonal antibody
DDX4,VASA Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 17545-1-AP is DDX4,VASA Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | mouse, rat |
Immunogen | DDX4,VASA fusion protein Ag11647 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 |
Synonyms | DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog |
Calculated Molecular Weight | 690aa,76 kDa; 724aa,79 kDa |
Observed Molecular Weight | 79 kDa |
GenBank Accession Number | BC047455 |
Gene Symbol | DDX4 |
Gene ID (NCBI) | 54514 |
RRID | AB_2918053 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NQI0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DDX4,VASA antibody 17545-1-AP | Download protocol |
IHC protocol for DDX4,VASA antibody 17545-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
- Journal Impact Factor
- Most recent
Species | Application | Title |
---|---|---|
Sci Rep FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells. |