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DDX4,VASA Monoclonal antibody, PBS Only

DDX4,VASA Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F12C3

Cat no : 67147-2-PBS

Print datasheet

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

验证数据展示

  • WB analysis of SKOV-3 using 67147-2-Ig (same clone as 67147-2-PBS)

    WB analysis of SKOV-3 using 67147-2-Ig (same clone as 67147-2-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    IHC staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    IF Staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody (67147-2-Ig, Clone: 1F12C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

产品信息

67147-2-PBS targets DDX4,VASA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DDX4,VASA fusion protein Ag11630 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Synonyms DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog
Calculated Molecular Weight690aa,76 kDa; 724aa,79 kDa
Observed Molecular Weight79 kDa
GenBank Accession NumberBC047455
Gene Symbol DDX4
Gene ID (NCBI) 54514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQI0
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].