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验证数据展示

  • WB analysis using 31211-1-AP

    WB analysis using 31211-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31211-1-AP (DDX42 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31211-1-AP

    IHC staining of mouse testis using 31211-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31211-1-AP (DDX42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31211-1-AP

    IF Staining of HeLa using 31211-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX42 antibody (31211-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

DDX42 Polyclonal antibody

DDX42 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31211-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

RNA helicase related protein, RNA helicase like protein, RHELP, EC:3.6.4.13, DEAD box protein 42

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31211-1-AP targets DDX42 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DDX42 fusion protein Ag34762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
别名 RNA helicase related protein, RNA helicase like protein, RHELP, EC:3.6.4.13, DEAD box protein 42
计算分子量 103 kDa
观测分子量130 kDa
GenBank蛋白编号BC015505
基因名称 DDX42
Gene ID (NCBI) 11325
RRIDAB_3669903
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86XP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX42 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 42) is a member of the DEAD box RNA helicase superfamily. A C-terminal portion of DDX42 interacts with the pro-apoptotic factor, apoptosis-stimulating protein of p53 protein 2 (ASPP2), thereby inhibiting its action (PMID: 30834153).

实验方案

Product Specific Protocols
WB protocol for DDX42 antibody 31211-1-APDownload protocol
IHC protocol for DDX42 antibody 31211-1-APDownload protocol
IF protocol for DDX42 antibody 31211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols