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DDX43 Monoclonal antibody

DDX43 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B3B2

Cat no : 68454-1-Ig

Print datasheet

Synonyms

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, DKFZp434H2114, HAGE, Helical antigen

验证数据展示

  • WB analysis using 68454-1-Ig

    WB analysis using 68454-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68454-1-Ig

    WB analysis using 68454-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68454-1-Ig

    WB analysis using 68454-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68454-1-Ig (DDX43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCalu-3 cells, LNCaP cells, HeLa cells, U2OS cells, DU 145 cells, NCCIT cells, rabbit testis tissue, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68454-1-Ig targets DDX43 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Rat, Rabbit
Immunogen DDX43 fusion protein Ag11484 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
Synonyms Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, DKFZp434H2114, HAGE, Helical antigen
Calculated Molecular Weight 648 aa, 73 kDa
Observed Molecular Weight 68-73 kDa
GenBank Accession NumberBC066938
Gene Symbol DDX43
Gene ID (NCBI) 55510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NXZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy.

实验方案

Product Specific Protocols
WB protocol for DDX43 antibody 68454-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols