验证数据展示
经过测试的应用
| Positive WB detected in | Calu-3 cells, LNCaP cells, HeLa cells, U2OS cells, DU 145 cells, NCCIT cells, rabbit testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68454-1-Ig targets DDX43 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Rat, Rabbit |
| 免疫原 | DDX43 fusion protein Ag11484 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 |
| 别名 | Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, DKFZp434H2114, HAGE, Helical antigen |
| 计算分子量 | 648 aa, 73 kDa |
| 观测分子量 | 68-73 kDa |
| GenBank蛋白编号 | BC066938 |
| 基因名称 | DDX43 |
| Gene ID (NCBI) | 55510 |
| RRID | AB_3085166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NXZ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX43 antibody 68454-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |