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验证数据展示

  • WB analysis using 85065-3-RR

    WB analysis using 85065-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85065-3-RR (DDX46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 85065-3-RR

    IF Staining of U-251 using 85065-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using DDX46 antibody (85065-3-RR, Clone: 242715E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85065-3-RR

    Affinity and Kinetic Characterization of 85065-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85065-3-RR against Human DDX46 were performed. The affinity constant is 51.9 pM.

DDX46 Recombinant antibody

DDX46 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85065-3-RR

产品说明书

CloneNo. 242715E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Probable ATP-dependent RNA helicase DDX46, KIAA0801, EC:3.6.4.13, DEAD box protein 46, 242715E10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, U-251 cells, NIH/3T3 cells, rat testis tissue
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85065-3-RR targets DDX46 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX46 fusion protein Ag10342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
别名 Probable ATP-dependent RNA helicase DDX46, KIAA0801, EC:3.6.4.13, DEAD box protein 46, 242715E10
计算分子量 1031 aa, 117 kDa
观测分子量140 kDa
GenBank蛋白编号BC012304
基因名称 DDX46
Gene ID (NCBI) 9879
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L014
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.

实验方案

Product Specific Protocols
WB protocol for DDX46 antibody 85065-3-RRDownload protocol
IF protocol for DDX46 antibody 85065-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols