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验证数据展示

  • WB analysis using 17795-1-AP

    WB analysis using 17795-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17795-1-AP (DDX47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 17795-1-AP

    IF Staining of U2OS using 17795-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (17795-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

DDX47 Polyclonal antibody

DDX47 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17795-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

DDX47, DEAD box protein 47, E4 DBP, HQ0256, MSTP162

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, SH-SY5Y cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17795-1-AP targets DDX47 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 DDX47 fusion protein Ag11953 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
别名 DDX47, DEAD box protein 47, E4 DBP, HQ0256, MSTP162
计算分子量 455 aa, 51 kDa
观测分子量48 kDa
GenBank蛋白编号BC068009
基因名称 DDX47
Gene ID (NCBI) 51202
RRIDAB_3085540
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0S4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).

实验方案

Product Specific Protocols
WB protocol for DDX47 antibody 17795-1-APDownload protocol
IF protocol for DDX47 antibody 17795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols