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验证数据展示

  • WB analysis using 83287-3-RR

    WB analysis using 83287-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83287-3-RR (DDX47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83287-3-RR

    FC experiment of U2OS using 83287-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX47 Recombinant antibody (83287-3-RR, Clone:240193C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of U2OS using 83287-3-RR

    IF Staining of U2OS using 83287-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U2OS using 83287-3-RR

    IF Staining of U2OS using 83287-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

DDX47 Recombinant antibody

DDX47 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83287-3-RR

产品说明书

CloneNo. 240193C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

MSTP162, HQ0256, EC:3.6.4.13, E4 DBP, DEAD box protein 47

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, SH-SY5Y cells, HEK-293 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83287-3-RR targets DDX47 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 DDX47 fusion protein Ag11953 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
别名 MSTP162, HQ0256, EC:3.6.4.13, E4 DBP, DEAD box protein 47
计算分子量 455 aa, 51 kDa
观测分子量45 kDa
GenBank蛋白编号BC068009
基因名称 DDX47
Gene ID (NCBI) 51202
RRIDAB_3670953
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H0S4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).

实验方案

Product Specific Protocols
WB protocol for DDX47 antibody 83287-3-RRDownload protocol
IF protocol for DDX47 antibody 83287-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols