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验证数据展示

  • WB analysis of NIH/3T3 using 10804-1-AP (same clone as 10804-1-PBS)

    WB analysis of NIH/3T3 using 10804-1-AP (same clone as 10804-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

    IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX5 antibody included
  • Featured Product
  • KD/KO验证

DDX5 Polyclonal antibody, PBS Only

DDX5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10804-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.

产品信息

10804-1-PBS targets DDX5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX5 fusion protein Ag1237 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
别名
计算分子量 69 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC016027
基因名称 DDX5
Gene ID (NCBI) 1655
RRIDAB_2292757
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17844
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation. For the reason that DDX5 can be phosphorylated and dephosphorylated at several sites, at some cases the molecular weight of DDX5 is less than 68kD, approximately 65kd