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DDX5 Polyclonal antibody

DDX5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26385-1-AP

Print datasheet

Synonyms

DDX5, DEAD box protein 5, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

验证数据展示

  • IP experiment of HeLa using 26385-1-AP

    IP experiment of HeLa using 26385-1-AP

    IP result of anti-DDX5 (IP:26385-1-AP, 4ug; Detection:26385-1-AP 1:500) with HeLa cells lysate 1600 ug.

  • IHC staining of mouse liver using 26385-1-AP

    IHC staining of mouse liver using 26385-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX5 antibody included
  • IHC staining of mouse liver using 26385-1-AP

    IHC staining of mouse liver using 26385-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26385-1-AP (DDX5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX5 antibody included
  • WB analysis of HepG2 using 26385-1-AP

    WB analysis of HepG2 using 26385-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26385-1-AP

    IF Staining of HepG2 using 26385-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (26385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26385-1-AP targets DDX5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Immunogen DDX5 fusion protein Ag24821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Synonyms DDX5, DEAD box protein 5, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68
Observed Molecular Weight 69 kDa
GenBank Accession NumberNM_004396
Gene Symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2880498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17844
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.

实验方案

Product Specific Protocols
WB protocol for DDX5 antibody 26385-1-APDownload protocol
IHC protocol for DDX5 antibody 26385-1-APDownload protocol
IF protocol for DDX5 antibody 26385-1-APDownload protocol
IP protocol for DDX5 antibody 26385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Eur J Med Chem

Discovery of potent thiazolidin-4-one sulfone derivatives for inhibition of proliferation of osteosarcoma in vitro and in vivo

Authors - Xuwen Chen
View Article