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DDX51 Polyclonal antibody

DDX51 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20149-1-AP

Print datasheet

Synonyms

EC:3.6.4.13, DEAD box protein 51, ATP-dependent RNA helicase DDX51

验证数据展示

  • WB analysis using 20149-1-AP

    WB analysis using 20149-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 20149-1-AP

    IP experiment of K-562 using 20149-1-AP

    IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:1000) with K-562 cells lysate 1820 ug.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Raji cells
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

20149-1-AP targets DDX51 in WB, IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DDX51 fusion protein Ag13691 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
Synonyms EC:3.6.4.13, DEAD box protein 51, ATP-dependent RNA helicase DDX51
Calculated Molecular Weight 666 aa, 72 kDa
Observed Molecular Weight 72-80 kDa
GenBank Accession NumberBC040185
Gene Symbol DDX51
Gene ID (NCBI) 317781
RRIDAB_10665947
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N8A6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The DEAD-box family (DDX) consists of a group of RNA helicases with the activity to hydrolyze ATP and to unwind RNA. The major physiological role of this family was to regulate RNA metabolism including ribosome assembly, RNA splicing, and pre-rRNA processing. DDX51 was a new gene in DDX family composed of 666 amino acids. DDX51 gene was a member of RNA helicases family in charge of regulating RNA metabolism(PMID: 31966432).

实验方案

Product Specific Protocols
WB protocol for DDX51 antibody 20149-1-APDownload protocol
IP protocol for DDX51 antibody 20149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

Authors - Tao Wang
View Article