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验证数据展示

  • WB analysis using 16660-1-AP

    WB analysis using 16660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16660-1-AP

    IF Staining of HepG2 using 16660-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16660-1-AP (DDX52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DDX52 Polyclonal antibody

DDX52 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16660-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Probable ATP-dependent RNA helicase DDX52, HUSSY-19, HUSSY19, EC:3.6.4.13, DEAD box protein 52

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16660-1-AP targets DDX52 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX52 fusion protein Ag10118 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 52
别名 Probable ATP-dependent RNA helicase DDX52, HUSSY-19, HUSSY19, EC:3.6.4.13, DEAD box protein 52
计算分子量 599 aa, 67 kDa
观测分子量 64 kDa
GenBank蛋白编号BC041785
基因名称 DDX52
Gene ID (NCBI) 11056
RRIDAB_1851052
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2R4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX52 antibody 16660-1-APDownload protocol
IF protocol for DDX52 antibody 16660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols