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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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0
Conjugate
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验证数据展示

  • WB analysis using 66664-1-Ig (same clone as 66664-1-PBS)

    WB analysis using 66664-1-Ig (same clone as 66664-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    IF Staining of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • WB analysis using 66664-1-Ig (same clone as 66664-1-PBS)

    WB analysis using 66664-1-Ig (same clone as 66664-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    IF Staining of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    FC experiment of PC-3 using 66664-1-Ig (same clone as 66664-1-PBS)

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human DDX54 (66664-1-Ig, Clone:1H9F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66664-1-PBS in a different storage buffer formulation.

DDX54 Monoclonal antibody, PBS Only

DDX54 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66664-1-PBS

产品说明书

CloneNo. 1H9F4

宿主/亚型

Mouse / IgG2a

种属反应性

mouse, human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:3.6.4.13, DP97, DDX 54, ATP-dependent RNA helicase DP97, ATP-dependent RNA helicase DDX54

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66664-1-PBS targets DDX54 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 mouse, human
免疫原 DDX54 fusion protein Ag25289 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
别名 EC:3.6.4.13, DP97, DDX 54, ATP-dependent RNA helicase DP97, ATP-dependent RNA helicase DDX54
计算分子量 98 kDa
观测分子量 98 kDa
GenBank蛋白编号BC156669
基因名称 DDX54
Gene ID (NCBI) 79039
RRIDAB_2882019
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDD1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.