验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells, U-251 cells, U-87 MG cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
30982-1-AP targets DDX60 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | DDX60 fusion protein Ag34406 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 |
| 别名 | Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60 |
| 计算分子量 | 1712 aa, 198 kDa |
| 观测分子量 | 170-200 kDa |
| GenBank蛋白编号 | BC038115 |
| 基因名称 | DDX60 |
| Gene ID (NCBI) | 55601 |
| RRID | AB_3669804 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8IY21 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX60 antibody 30982-1-AP | Download protocol |
| IHC protocol for DDX60 antibody 30982-1-AP | Download protocol |
| IF protocol for DDX60 antibody 30982-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Inflamm Res Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis | ||
Front Cell Dev Biol Genome-wide analysis reveals the MORC3-mediated repression of PD-L1 expression in head and neck cancer |
