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DEDD2 Polyclonal antibody

DEDD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14574-1-AP

Print datasheet

Synonyms

DED containing protein FLAME 3, DEDD2, FLAME 3, FLAME3

验证数据展示

  • WB analysis of HEK-293 using 14574-1-AP

    WB analysis of HEK-293 using 14574-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14574-1-AP (DEDD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14574-1-AP

    IHC staining of human brain using 14574-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 14574-1-AP

    IHC staining of human brain using 14574-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14574-1-AP targets DEDD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DEDD2 fusion protein Ag5724 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name death effector domain containing 2
Synonyms DED containing protein FLAME 3, DEDD2, FLAME 3, FLAME3
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC027930
Gene Symbol DEDD2
Gene ID (NCBI) 162989
RRIDAB_2091538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DEDD2 antibody 14574-1-APDownload protocol
IHC protocol for DEDD2 antibody 14574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.

Authors - Leah Fitzsimmons
View Article