验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human small intestine tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DEFA1 Ready-To-Use IHC Kit. DEFA1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18057-1-AP targets DEFA1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | DEFA1 fusion protein Ag12592 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | defensin, alpha 1 |
| 别名 | HP 1-56, HNP-1, defensin, alpha 1, DEFA2, DEFA1B |
| 计算分子量 | 94 aa, 10 kDa |
| 观测分子量 | 6 kDa |
| GenBank蛋白编号 | BC093791 |
| 基因名称 | DEFA1 |
| Gene ID (NCBI) | 1667 |
| RRID | AB_2292842 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P59665 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DEFA1 antibody 18057-1-AP | Download protocol |
| IHC protocol for DEFA1 antibody 18057-1-AP | Download protocol |
| IF protocol for DEFA1 antibody 18057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1. | ||
Hum Cell Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer. | ||
Syst Biol Reprod Med Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts. | ||
Mediators Inflamm Potential Association of Gut Microbial Metabolism and Circulating mRNA Based on Multiomics Sequencing Analysis in Fetal Growth Restriction |
