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验证数据展示

  • WB analysis of human peripheral blood leukocyte using 67156-1-Ig

    WB analysis of human peripheral blood leukocyte using 67156-1-Ig

    Human peripheral blood leukocyte cells were subjected to SDS PAGE followed by western blot with 67156-1-Ig (DEFA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 67156-1-Ig

    IHC staining of human small intestine using 67156-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:2000 (under 10x lens).

    Ready-to-use IHC kits available with DEFA1 antibody included
  • IHC staining of human small intestine using 67156-1-Ig

    IHC staining of human small intestine using 67156-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67156-1-Ig (DEFA1 antibody) at dilution of 1:2000 (under 40x lens).

    Ready-to-use IHC kits available with DEFA1 antibody included
  • IF Staining of mouse spleen using 67156-1-Ig

    IF Staining of mouse spleen using 67156-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using DEFA1 antibody (67156-1-Ig, Clone: 1A2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DEFA1 Monoclonal antibody

DEFA1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67156-1-Ig

产品说明书

CloneNo. 1A2B3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

HNP-1, defensin, alpha 1, DEFA2, DEFA1B, DEFA 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuman peripheral blood leukocyte cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy DEFA1 Ready-To-Use IHC Kit. DEFA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67156-1-Ig targets DEFA1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 DEFA1 fusion protein Ag12592 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 defensin, alpha 1
别名 HNP-1, defensin, alpha 1, DEFA2, DEFA1B, DEFA 1
计算分子量 94 aa, 10 kDa
观测分子量 6-10 kDa
GenBank蛋白编号BC093791
基因名称 DEFA1
Gene ID (NCBI) 1667
RRIDAB_2882453
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP59665
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DEFA1 antibody 67156-1-IgDownload protocol
IHC protocol for DEFA1 antibody 67156-1-IgDownload protocol
IF protocol for DEFA1 antibody 67156-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols