验证数据展示
经过测试的应用
Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18268-1-AP targets DEFA5 in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | DEFA5 fusion protein Ag12793 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | defensin, alpha 5, Paneth cell-specific |
别名 | DEF5, DEFA5, Defensin 5, Defensin, alpha 5, defensin, alpha 5, Paneth cell, HD 5 |
计算分子量 | 94 aa, 10 kDa |
GenBank蛋白编号 | BC069690 |
基因名称 | DEFA5 |
Gene ID (NCBI) | 1670 |
RRID | AB_2878526 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q01523 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEFA5, also named as DEF5, belongs to the alpha-defensin family. It has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Mature DEFA5 is about 5kd. It has a homodimer form.
实验方案
Product Specific Protocols | |
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IHC protocol for DEFA5 antibody 18268-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |