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DEM1/EXO5 Polyclonal antibody

DEM1/EXO5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25991-1-AP

Print datasheet

Synonyms

C1orf176, DEM1, EXO5, Exonuclease V

验证数据展示

  • WB analysis using 25991-1-AP

    WB analysis using 25991-1-AP

    NIH/3T3 cells lysates were subjected to SDS PAGE followed by western blot with 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 25991-1-AP

    IF Staining of A431 using 25991-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DEM1/EXO5 antibody (25991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25991-1-AP targets DEM1/EXO5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DEM1/EXO5 fusion protein Ag22666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name defects in morphology 1 homolog (S. cerevisiae)
Synonyms C1orf176, DEM1, EXO5, Exonuclease V
Observed Molecular Weight42 kDa
GenBank Accession NumberBC021969
Gene Symbol DEM1
Gene ID (NCBI) 64789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
IHC protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
IF protocol for DEM1/EXO5 antibody 25991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols