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DENND1A Polyclonal antibody

DENND1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25658-1-AP

Print datasheet

Synonyms

Connecdenn, DENN/MADD domain containing 1A, DENND1A, FAM31A, KIAA1608, Protein FAM31A, RP11 230L22.3

验证数据展示

  • WB analysis of SKOV-3 using 25658-1-AP

    WB analysis of SKOV-3 using 25658-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 25658-1-AP

    IHC staining of human endometrial cancer using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 25658-1-AP

    IHC staining of human endometrial cancer using 25658-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25658-1-AP (DENND1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of K-562 using 25658-1-AP

    IP experiment of K-562 using 25658-1-AP

    IP result of anti-DENND1A (IP:25658-1-AP, 4ug; Detection:25658-1-AP 1:600) with K-562 cells lysate 3200ug.

  • WB analysis using 25658-1-AP

    WB analysis using 25658-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25658-1-AP (DENND1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of SKOV-3 using 25658-1-AP

    IF Staining of SKOV-3 using 25658-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using DENND1A antibody (25658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inSKOV-3 cells, A2780 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman endometrial cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25658-1-AP targets DENND1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen DENND1A fusion protein Ag22547 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DENN/MADD domain containing 1A
Synonyms Connecdenn, DENN/MADD domain containing 1A, DENND1A, FAM31A, KIAA1608, Protein FAM31A, RP11 230L22.3
Calculated Molecular Weight 1009 aa, 111 kDa
Observed Molecular Weight 111 kDa, 64 kDa
GenBank Accession NumberBC039703
Gene Symbol DENND1A
Gene ID (NCBI) 57706
RRIDAB_2880180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEH3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DENND1A (DENN/MADD domain containing 1A), also known as Connecdenn,FAM31A or KIAA1608, is a 1,009 amino acid peripheral membrane protein. Recent studies has found DENND1A is strongly associated with PCOS (Polycystic ovary syndrome). DENND1A has several isoforms.This antibody(25658-1-AP) can recognize all the isoforms at 112 kDa, 52-64 kDa.

实验方案

Product Specific Protocols
WB protocol for DENND1A antibody 25658-1-APDownload protocol
IHC protocol for DENND1A antibody 25658-1-APDownload protocol
IF protocol for DENND1A antibody 25658-1-APDownload protocol
IP protocol for DENND1A antibody 25658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols